Archive List for Physical Chemistry

HPLC: Methods and Principles of Reversed-Phase Chromatography

Chromatography is an experimental operation for the separation of substances. One of the most common experimental operations in chromatography is HPLC (high performance liquid chromatography). High performance liquid chromatography is used in almost every laboratory, including physical chemistry, organic chemistry, and biochemistry. Although the experiments performed vary from lab to lab, HPLC is the analytical […]

Absorbance of UV-Vis Absorption Spectrum: Beer–Lambert Law

A large number of laboratories use absorbance when analyzing in experimental manipulations. By measuring how much light passes through, you can measure how much of a substance is present in the solution. Alternatively, it can be used to check how far a reaction has progressed. When experimenting with biological systems, we frequently use absorbance to […]

Column Chromatography: Separating Compounds and Determine the Developing Solvent

Chromatography is a method of separating substances completely. There are several types of chromatography, of which column chromatography is the most frequently used in laboratories that deal with organic compounds. A type of adsorption chromatography is column chromatography. It uses an adsorbent called silica gel to separate several organic compounds that are mixed together. Column […]